R1b-M269 and the Steppe Eneolithic.

In 2019 Wang et al. published three ancient samples from north Caucasian foothill steppe region. They got the label Steppe Eneolithic. The term "Steppe" is usually used in genetic papers to label DNA related or similar to Yamnaya genetic profile.

Those Steppe Eneolithic samples were remarkable because not only they have the genetic profile similar to Yamnaya but were also older than it. Back then they didn't get the attention they deserved because they were not from a famous culture. In 2024 Lazaridis et al. proposed a new name for them the Caucasus - Lower Volga cline and added new similar samples. Genetic calculations showed that genome wide Yamnaya got most of their ancestry from this CLV folks. Despite this advance the two most important Y markers were still not securely linked to this CLV population. The R1b-M269 and R1a-M417 which had a large expansion all over Eurasia spreading the Indo-European languages.

Ghalichi et al. 2024 filled this gap. Two new cases of R1b-M269 were found before the Yamnaya near North of Caucasus region. They had similar to other Steppe Eneolithic groups ancestry dated at around 3800BC. It's worth to note that there was another older M269 sample (I33307) from Kalmykia dated around 3700 but it's genetic profile is identic to Core Yamnaya raising the suspicion that it is younger than the radiocarbon dates show. Lazaridis et al. labeled this sample as Russia_Caspianinland_EBA_Yamnaya group that is why I didn't included it in this list of Steppe Eneolithic Y DNA (4700-3000BC. See the map for the geographic area)

R1b-V1636 -----9

R1b-M269. -----2

R1a. -----1

I2-L699. ------1

J2a ------1

J2b2a. ------1

Besides those cases another possible old R1b-M269 (around 4500BC) was found in Bulgaria in Varna culture burial. It's autosomes showed an obvious Steppe Eneolithic related ancestry. Which means that it was not related to local Neolithic farmers and was a migrant from the Caucasus Lower Volga region. Varna was a peculiar culture with highly hierarchical organization. Large number of gold items were found from Varna culture graves.

Another important finding in the Steppe Eneolithic context is off course the J2b2a-L283. Its autosomes were more Caucasus shifted which is understandable. But overall, it was part of that emerging kurgan Eneolithic groups from which the Yamnaya and Corded Ware Culture will stem few centuries later.

We haven't yet found the R1a-M417 in the forementioned context. But given that one of oldest R1a-M198 (close to M417) was found in Middle Volga region we can assume that M417 will be found in Steppe Eneolithic / CLV region also. Currently one of oldest R1a-M417 is from Yamnaya layer in Balkans.

Based on this data I will restate my scepticism about the possibility that Yamnaya descend from Sredny Stog located in Ukraine expressed on some boards. Highest diversity of Z2103+ and negative to Z2106 is found in Near East which means that the region from which Z2103 started its expansion must be close to Caucasus. More ancient DNA will hopefully solve this question also.

PS. I didn't include in that Eneolithic Y DNA list samples from Steppe Maykop who have a different Central Asian origin. Also, one of V1636 is dated at 2800BC which is later than the 3000BC. But its autosomes shows that it was from preceding period and not from Core Yamnaya.

See also

• Qpadm models about the Core Yamnaya origins.
• The Yamnaya culture and it's legacy.

The Kura-Araxes culture. An open thread.

Ghalichi et al. 2024 have published 7 new KA ancient samples from Georgia. Site Dzedzevbi near Dmanisi. And two more samples from Velikent Dagestan.

The low coverage didn't permit to find the deep subclades of those samples. But apparently two male samples from Dzedzevbi are J2b2. While the other is J1 almost certainly from the Z1842 branch. The Velikent is also J1.

Overall, there is now 16 Y DNA from known Kura-Araxes layers. 11 of them are J1 from the Z1842 branch. Most cases of J1 are found in regions that are geographically close to eastern Great Caucasian range. Dagestan, Kakhetia, Berkaber in Tavush and one case near Dmanisi. The strong prevalence of J1 is almost certainly the result of founder effect, because in earlier Neolithic periods the J1 was rare.

Moving away from the eastern parts of Caucasian range we see other haplotypes. Like R1b-V1636 from Sevan basin, G2b from Kaps Shirak. J2-M92 from Doghlauri central Georgia. And now two cases of J2b2 from Dzedzevbi. According the Genarchivist activists one of them is from the J2b-FT3464 a minor branch found today in Near East and Europe. While the other J2b was J2b2b-Z2453. An old Neolithic branch found in Shulaveri culture and Hajji Firuz tepe.

What can we deduce from this distribution? As I have already noted the J1 in northeastern parts of Kura-Araxes horizon can be associated with North-East Caucasian (NEC) speakers. But the whole KA horizon couldn't have been NEC speakers otherwise this would have left linguistic traces. For which there is no evidence. The rapid shift in Y DNA distribution when moving away from eastern Caucasus is another strong argument that there was another ethnic group (or groups) in Kura-Araxes.

Who could be this other group(s)?

The two main candidates are the Anatolian speakers and Hurro-Urartians. Currently the data is still too small to connect the dots between South Caucasus and Anatolia/Levant/Mesopotamia where Anatolian Hurro-Urartian languages were spoken. But some patterns are already visible.

See also

• Here is an excerpt from the Petrosyan 2023 paper about the origins of Kura-Araxes culture.
• 
  

I2a2b-Y16419 a possible Ukraine hunter gatherer lineage in Yamnaya/Catacomb?

Lazaridis et al. 2024 had discovered that Yamnaya has some Ukraine Neolithic hunter gatherer ancestry. UNHG. This term can be confusing but it's a result of different naming convention in ex-Sovietic countries, when a hunter-gatherer community gets the Neolithic label if they had a pottery.

Ghalichi et al. 2024 confirmed the presence of some 15% of UNHG in Yamnaya. The I2-L699 regularly found in steppe was derived from UNHG for example.

The presence of I2 in Yamnaya related cultures is an important subject for the Armenian ethnogenesis given the presence of I2a2b in Trialeti-Vanadzor culture.

Till now we didn't have any extra information about this haplotype. The closest European sample to it was found in Eneolithic Croatia with a common ancestor reported to live at 8200BC by the FTDNA. This age is amply sufficient for this lineage to be present both in European farmers and in UNHG.

Ghalichi et al. 2024 had a new sample which probably can help to understand how I2a2b could end up in early Yamnaya communities as a rare lineage. The paper reported a single female sample from near Azov and Black Sea joining region labeled as "Steppe Eneolithic outlier west". KHB dated at 4000BC near Taman peninsula. This sample has large amount of UNHG ancestry (43%). In most likelihood it infiltrated there from Ukraine.

If this outlier represents a regular population living in that region, then this raises the possibility that the I2a2b-Y16419 was also living, there since the Eneolithic and with the formation of Yamnaya/Catacomb became part of those cultures as a rare lineage. Later it moved to south and had a great luck to expand with Trialeti-Vanadzor culture.

For verifying this theory more ancient DNA is needed from the aforementioned region in particular and from south Ukraine in general.

Shulaveri-Aratashen-Shomutepe (SAS) culture. 7000/6200-5300BC.

 Shulaveri-Aratashen-Shomutepe (SAS) culture. 7000/6200-5300BC.

We have five good quality new samples from Shulaveri (SAS) culture in Georgia (Aruchlo). They had YDNA H2, J2a1a and R. This latter is from the R2 haplogroup according Genarchivist activists.

On the PCA three of five farmers plot close to related Neolithic samples from Armenia and Azerbaijan while modern people close them are the Armenians in G25. It's now obvious that this was the main genetic profile in SAS/Shulaveri culture. Those were the first farmers in South Caucasus and their ancestry was largely derived from central regions of Fertile Crescent hence the reason that occasionally we call them Central farmers. Ghalichi 2024 used also the term East Anatolian farmers. Given some archaeological data from Van region we can assume that most of historic Armenia (except probably the most western and southwestern regions) was inhabited by this type of farmers.

Besides this "Central"/"Armenian like" type there were also farmers with higher CHG ratio. First we have seen them in Aknashen from Armenia. Now we have another similar CHG shifted sample from Georgia plotting close to modern Georgians. It's not exactly identic to Aknashen but rather plots close to Darkveti-Meshoko ( labeled as Caucasus Eneolithic ) raising the possibility that Darkveti-Meshoko culture formed as a mixture of Shulaveri and CHG. The Darkveti culture is remarkable because genome wide it's genetic profile looks a good candidate for being Pre-Proto-Kartvelian. High CHG and very low Steppe. But the absence of G2a1a there and scant sampling from west Caucasus makes those suggestions still speculative.

Another sample from Aruchlo/Georgia plots close to CHG hunters. The ARO006 with YDNA R2. Making it a hunter who learned farming without having any significant admixture from those farmers. The presence of such hunter related genetic profile in Shulaveri culture means that there were at last two different languages in SAS. One derived from the first farmers and another derived from the hunters who learned farming. It's remarkable that archaeology supports this dualistic nature of Shulaveri culture. Two different potteries were made in Shulaveri. One of them was Chaff-tempered. The other one was Grit-tempered. Chaff-tempered was almost certainly made by the first farmers who came from southern regions of historic Armenia. While the Grit-tempered was made by groups derived from the local hunters.

It's interesting that both pottery traditions continued in ancient South Caucasus and historic Armenia after the Neolithic period. Grit-tempered was prominent in Sioni (also found in Adablur and Guinchi) culture which evolved in Early and Middle Chalcolithic (5300-4300BC). . We can conjecture that they were CHG shifted. Offcourse this is a just a prediction based on archaeology which can be wrong, given that currently there are no samples from this period. While Chaff-tempered pottery became prominent in Late Chalcolithic period (4300-3600BC). The Late Chalcolithic period DNA both from Armenia and Azerbaijan shows that they were mostly derived from the first Neolithic farmers having some extra new admixtures.

And finally we can now say with high degree of certitude that the CHG shifted genetic profile of Kura-Araxes culture (3600-2400BC) had local origins. When farmers settled all over Kur and Arax river valleys the forest-mountain zone between those two valleys also known as Lesser Caucasus became a sort of refugium where they preserved the initial hunter gatherer ancestry in higher proportion. This is the reason why the oldest radiocarbon dated Kura-Araxes sites are found in north Armenia (Gegharot) and south Georgia.

But this is not the whole story. Apparently Kura-Araxes also had two potteries and genetic profiles. Which could mean that for at last 4000 years two genetic profiles were competing in South Caucasus starting from the Neolithic period till the end of Early Bronze Age.

PS. There was in reality six samples from Aruchlo. But one of them has so little coverage that it was not included in the calculations.

Ghalichi et al. 2024 and the Indo-European question.

 Ghalichi et al. 2024 and the Indo-European question.

A new paper with more than 100 samples provided new details about the interaction of Eastern European populations and West Asians over Caucasus.

A new Mesolithic sample from north Caucasus (Satanae cave SJG001) dated at 6100BC yielded an interesting genetic profile. It was practically identic to other Eastern European hunter gatherers (EHG). The sample had the R1a haplogroup, from a minor branch (not the same as M417) that in most likelihood is related to later IE migrations.

This EHG result from north Caucasus means that the genetic profile of Yamnaya didn't existed since the immemorial times but formed later than the 6000BC. Yamnaya has ancestry both from EHG and from south of Caucasus. The event that triggered the formation of Yamnaya better known as Steppe genetic profile was the apparition of farmers in South Caucasus. The Shulaveri-Aratashen-Shomutepe (SAS) culture starts no later than 6200BC. And starting from this period farmers mixed both with Caucasian hunter gatherers and made attempts to colonize the steppe in north of Caucasus.

A new case of J2b2a-L283 was found in steppe zone of North Caucasus (the ZO1002 on the map). It was dated at 3800BC, older than Maykop and Kura-Araxes. And already had some EHG admixture which means that it was probably a lineage descending from Shulaveri farmers trying to settle in steppe. Later this haplotype will become a rare Yamnaya lineage and will move to Balkans where it will have an important expansion.

Despite this finding the most predominant Y DNA in Eneolithic steppe pastoralistic groups had local Eastern European origin (R1 and I2) rather than from south of Caucasus. We have another Y DNA from Nalchik farmers and this time once more it's R1b. From the V1636 branch. We have also now one of oldest R1b-M269 from Konstantinovka dated at 3800BC. KST001.

So two important question arises..

Why despite having large amount of West Asian/Caucasian ancestry the Eneolithic steppe groups and Yamnaya has so little cases of J haplogroup.

And the second question is linguistic. Who's language was the Proto Indo Anatolian? Those from Shulaveri culture farmers or those from EHG / R1 side.

Before answering the first question we should note that this phenomenon of mismatch between autosomes occurred not only in East Europe but also in West Europe. After the initial success of G2 haplogroup , later there was a widespread resurgence of local I2 haplogroup. In British islands farmers for instance had 100% of I2 haplogroup. Yet in autosomes they had 70% of Anatolian farner ancestry.

There can be numerous theories to explain this. I am personally inclined to believe that social factors played a key role. Neolithic farmers of West Asia had more egalitarian or even matriarchal culture. A recent paper from Catalhoyuk showed an obvious matrilineal culture. While eastern European hunters could have more patriarchal culture. When a matriarchal and patriarchal cultures meet each other this affects in different ways the uniparental markers. Y DNA of one side will prevail and mtdna of the other side will also prevail.

There are other theories like mass killing of males. Those theories are popular in internet. But they can't explain one important thing. Why Scythians, Saka and Turkic tribes who are known to have a warrior culture and where frequently killing all males of the competing clan had one of most diverse Y DNA. The answer is self evident. Because during a conflict it's not granted that always one haplogroup prevails while others always are defeated. The outcome of the clans conflict can be very random which will create a very random distribution of haplogroups.

When farmers moved to north of Caucasus they were the technologically advanced side. It would be strange that they lost all battles without wining any of them and expanding their lineage. So wars and mass killings can't explain the emergence of this pattern. There can be other explanations. I just demonstrated one possible.

As for the linguistic part I will dedicate another topic