The Eastern-Side Armenians
Hovhannisyan et al. (2024) published numerous DNA samples from the Artsakh, Syunik, and Gardman regions. In addition, a member of our group, Diana Sarkisova Vikutan, provided several coordinates from Shamakhi Armenians, as well as DNA samples with mixed ancestry from the Shamakhi and Artsakh regions. We are grateful to her for sharing these data.
All these datasets were combined into a single PCA and ancestry proportion chart together with other modern and ancient samples spanning the area from Syunik to Shamakhi. The analysis was performed using unscaled coordinates. Based on this, several observations can be made:
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Artsakh and Syunik Armenians: Artsakh Armenians are genetically very similar to Syunik Armenians. Both belong to the genetic cline of historic Armenia, which shows a strong affinity with the so-called “Central farmer” ancestry. Gardman Armenians are also close to this cluster, though with a slight shift toward Caucasus hunter-gatherer (CHG) ancestry.
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Distance from Armenia LIA samples: All Armenian subgroups plot away from the Armenia LIA samples from Syunik. This is due to a so-called “southern” shift that may have occurred in these eastern regions sometime after the fall of Urartu.
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Shamakhi Armenians: Shamakhi Armenians show higher steppe ancestry than any other known Armenian subgroup. The two samples from the Shamakhi region differ somewhat from each other. One of them (Sham01) displays more Zagros Neolithic ancestry. The source of this shift is currently unclear. It is worth noting that a Late Antiquity sample (3rd century AD) from the Shamakhi region already showed extra Zagros/Iran Neolithic ancestry, almost certainly due to Iranian influence in that region. Another possibility is that the Zagros shift in Sham01 reflects ancestry from a more recent non-Armenian population. Yet another possibility is that the shift derives from a still poorly sampled subgroup of Tat Armenians.
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Mixed Shamakhi–Artsakh samples: Three DNA samples show mixed Shamakhi and Artsakh ancestry. As expected, they plot between the Shamakhi and Artsakh clusters. However, their dispersion suggests that the real diversity among Shamakhi Armenians could be greater than what the currently available two samples indicate.
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Udi population: The Udi people plot far from their linguistic relatives, the Lezgins. Moreover, they are also shifted away from the Late Antiquity Shamakhi sample that may represent Caucasian Albanian ancestry. The formation of the Udi genetic profile is still unclear, but their proximity to Armenians—who themselves are shifted toward the southwest—suggests that Udis may have acquired Armenian ancestry either in antiquity or, more likely, during the medieval period. It is also noteworthy that some Azerbaijanis from the Shaki region plot close to Udis, indicating that the influence of Armenian-like ancestry was not limited to the Udi population.
Additionally, the Skourtanioti et al. (2024) paper noted an “Anatolian” shift in Iberia and linked it to the spread of Christianity. It is possible that similar processes occurred in Caucasian Albania during Late Antiquity and the Early Medieval period. Hopefully, with the publication of a temporal transect from the Kakhetia region, we will gain a better understanding of how the genetics of Caucasian Albania evolved during historical times.
In summary, the genetic history of the territory of the modern Azerbaijani Republic appears to have become quite complex after the Bronze and Iron Ages. Multiple influences shaped the genetic diversity of the region. The single Caucasian Albanian sample remains somewhat puzzling: it is still unclear whether it represents typical Albanian ancestry or an outlier. As usual, more modern and ancient samples from these regions are needed to better understand their genetic history.



